EXPRESSION PATTERN OF AtHKT1 IN Arabidopsis thaliana
abstract:
AtHKT1 has been reported as a Na transporter. However, the membrane localization and gene expression regulation of the AtHKT1 in Arabidopsis thaliana remain unknown. Here, we performed immunological staining on AtHKT1 with an anti-AtHKT1 antibody and analyzed the gene expression pattern of GUS fusions with the AtHKT1 promotor, and over-expressed/distrupted AtHKT1 lines to understand the morphology, anatomy and expression of AtHKT1 transporter in relation with induction of the osmolarity and phytohormone.
The immunoblotting and histochemical analysis of GUS-fused AtHKT1-expressing Arabidopsis shown that AtHKT1 was expressed in the leave cells around vascular tissues, as well as in trichome. The distruption of AtHKT1 reduced the number of trichome and distance of among tissues in leaves. The size of AtHKT1 mutant plants which enter generative stage earlier, was smaller than that of wild type and overexpressor.
Expression of AtHKT1 was induced by salt and osmolarity stress. The GUS activity assay was increased proportionally with increasing concentration of Na and K, and reached maximum values when the plants were supplied with 30 mM Na or K. The expression level of GUS gene also increased with the increasing concentration of sorbitol or mannitol, and reached maximum values when the plants were supplied with 100 mM sorbitol or mannitol, eventhough at this condition the growth of plants was inhibited. The expression of AtHKT1 in response to gibberellin (GA3) was slightly higher than in plants treated with IAA, BAP, kinetin, zeatin, methyl jasmonate and brassinolide. The strongest activity has been observed in plants treated with NAA. The expression of AtHKT1 is regulated by kind of phytohormone.
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